Gcn5 transcriptional activation of Myc-induced genes promotes B-cell lymphomagenesis

Purpose: In this study we sought to elucidate whether Gcn5 cooperates with Myc to induce the formation of lymphoma using an in vivo model, the Eµ-Myc mouse. In order to define the molecular mechanisms for how Gcn5 contributes to lymphoma development upon overexpression of Myc, we performed RNA-seq analyses on total RNA isolated from CD19+ B cells sorted from spleens of 5-6-week-old wild-type, Eµ-Myc, and Eµ-Myc; Gcn5Fx/Fx mice. Conclusions: Loss of Gcn5 not only ameliorates dysregulation of the cell cycle caused by Myc overexpression, but also affects Myc-induced pathways related to cancer cell growth. mRNA profiles of CD19+ B cells of 5-6 week old WT, Eµ-Myc, and CD-19 Cre; Eµ-Myc; Gcn5 Fx/Fx and mice.