File S1 - A New Approach for the Study of Lung Smooth Muscle Phenotypes and Its Application in a Murine Model of Allergic Airway Inflammation

All supporting materials, including supplementary methods, tables, and figures are included in File S1. Table S1 lists all primer sequences for real-time PCR. Table S2 lists all deregulated gene list in BSMCs from OVA sensitized mice. Figure S1 shows the sorting plot of dissociated lung cells. Figure S2 shows post-sort purity tests for sorted hrGFP+ and hrGFP+DsRed+ cells from the lungs of αSMA-hrGFP+;NG2-DsRed+ mice. Figure S3 shows the examination of appropriate marker expression in sorted cell population. Figure S4 shows characterization of physical properties of BSMCs from a mouse model of chronic asthma. Figure S5 shows the concordance of our BSMCs array results with sets of genes identified as differentially expressed by previous asthma-related studies. (PDF)