Comparative Quantitative Phosphoproteomic Analysis of the Chicken Egg during Incubation Based on Tandem Mass Tag Labeling

Protein phosphorylation plays an important role in protein
structure
and function. To investigate the role of egg protein phosphorylation
in chicken embryonic development, a comparative and quantitative phosphoproteomic
analysis of fertilized chicken egg white and yolk was performed during
incubation. Overall, 215 phosphosites mapped onto 205 phosphopeptides
corresponding to 100 phosphoproteins were identified. Among these
phosphoproteins, 123 phosphosites from 62 egg proteins were found
significantly changed (p < 0.05) at day 12 during
incubation. Furthermore, GO analysis suggested that these differentially
phosphorylated proteins were associated with various molecular functions,
primarily including binding, molecular function regulator, and transport
activity. Such findings in this study improved our understanding of
the protein molecular functions involved in chicken embryonic development
from a protein phosphorylation perspective.