Tammar & parma wallaby GBS data from NZ

While conducting a landscape genomics study of invasive tammar wallabies (Notamacropus eugenii) in Aotearoa New Zealand we discovered that parma wallabies (N. parma) are also present in the North Island. This population has gone undetected for at least 30 years (and potentially for over a century), hidden amongst the morphologically similar tammar wallabies. The fact that an invasive wallaby species could remain undetected for so long, highlights the need for greater monitoring efforts for invasive species including genomic species identification., Laboratory and bioinformatic methodsWe used a DNeasy blood and tissue extraction kit on a QiaCube to extract the DNA from c. 0.5 cm2 of tissue, with an overnight digest using proteinase K according to the manufacturer’s protocols (QIAGEN, Hilden, Germany). DNA was eluted into 200 μL of Buffer AE and then stored at − 20 °C. The quality and quantity of the DNA were evaluated using a denovix DS-11 nucleic acid spectrophotometer, examining the 260/230 and 260/280 ratios to determine if there was any contamination. Any sample that did not meet the criteria for purity (260/280 = 1.7–2.1, 260/230 = 1.9–2.2) were removed.
All DNA extractions were then diluted to a uniform 50 ng/μL (with a concentration step using a SpeedVac for samples that had low concentration), with 1 μg of DNA sent for each sample for GBS sequencing. This GBS was performed at GenomNZ Animal Genomics Group (AgResearch, New Zealand). Procedures followed Dodds et al. (2015) after Elshire et al. (2011), with the following modif..., , # Tammar & Parma Wallaby GBS data from NZ

[https://doi.org/10.5061/dryad.80gb5mkzz](https://doi.org/10.5061/dryad.80gb5mkzz)

## Description of the data and file structure

Metadata for each sample including ID, Species, Date, Sex, Latitude, and Longitude, as well as the first 4 Principal Coordinates can be found in the .csv file.  

The VCF file contains the filtered SNP data for all samples.
## Code/software

No specific software is required, the csv can be read in any text editor or database software.  The vcf can be read in any text editor and can be analysed with many packages such as vcftools, bcftools, plink etc.