Open chromatin in somatic cells transplanted into Xenopus oocytes

Active gene transcription is associated with an open chromatin configuration at gene regulatory regions. However, the relationship between chromatin architecture of somatic cells and transcriptional activation during nuclear reprogramming in oocytes is unclear. We therefore examined changes in open chromatin states before and after transplantation of mouse somatic nuclei into Xenopus laevis oocytes by using the Assay for Transposase-Accessible Chromatin Sequencing (ATAC-seq). ATAC-seq was performed using the following cell samples; (1) C2C12 cells, (2) mouse embryonic fibroblasts (MEFs) before and after nuclear transplantation into Xenopus laevis oocytes.