Single cell RNA-sequencing of Spike-ins and mESC using Smart-Seq2 on C1 System. Single cell RNA-sequencing of Spike-ins and mESC using Smart-Seq2 on C1 System

"In this study, we assess technical differences between commonly used single-cell RNA-Sequencing (scRNA-Seq) methods. We perform scRNA-seq on a homogenous population of mouse embryonic stem cells along with two kinds of control spike-in molecules to assess sensitivity and accuracy of these specific methods. In this dataset, we perform Smart-Seq2 method on Fluidigm C1 system and generate single-cell libraries using Nextera XT kit"