Proteins with a predicted N-terminal signal peptide identified exclusively in the secretome of E. faecalis grown on urine.

Domain structures were identified using Pfam and shown in S1 Table. EF0577 and EF2076 contains TroA domains, which are part of a periplasmic solute binding protein family; part of a family of ABC transporter metal-binding lipoproteins. EF0063 and EF3106 contains SBP_bac_5 domains, which are bacterial extracellular solute-binding proteins, believed to be involved in active transport of solutes across the cytoplasmic membrane. EF3054 contains two PepSY domains, which are thought to have a protease inhibitory function. For EF0764, Pfam does not find any known domains, nor does it annotate a signal peptide as with the other proteins, however both SignalP, LipoP and THMM indicate the presence of such a signal peptide. The functional annotation provided by Gene Ontology (GO) is based on inferences from the domain annotations (InterPro2GO). Additional information on these proteins is available in Table 2 and S1 Table.Proteins with a predicted N-terminal signal peptide identified exclusively in the secretome of E. faecalis grown on urine.