Bacterial community changes in casing material during Grifola frondosa wood log cultivation in the forest.

In our previous study, although Grifola frondosa biomass was not detected in casing material, most of the microbiome in wood logs was derived from casing material. Therefore, understanding the composition of casing material bacterial communities, and the interaction between maitake and casing material bacterial communities is important in maitake wood log cultivation. The casing material is a coverage material put onto the substrate spread by the mushroom mycelium. Different from commercial button mushroom cultivation using sterilized soil in an artificial environment, in maitake wood log cultivation, people use mountain sand or soil obtained from the neighborhood to bury wood logs in the forest. However, does this kind of artificial disturbance harm casing material biological activity or cause soil degradation. It has been reported that more than 30 percent of the soil is degraded by various disturbances, and more than 20 percent of irrigated land is faced with salinization problems. Moreover, disturbances in the soil environment negatively affect soil properties and microbial diversities in the soil. Therefore, these issues must be taken seriously because maitake cultivation casing material will remain in the cultivated place in the forest's ecological environment. The purpose of this study is to understand the dynamics of bacterial communities in casing material, and assess the effect of maitake cultivation disturbance on casing material in the forest.