Emerging infection and nosocomial transmission of carbapenem-resistant hypervirulent Klebsiella pneumoniae harboring blaNDM-21 in a neonatal intensive care unit

Dataset Short DescriptionThe dataset contains complete genome sequences, plasmid maps, antimicrobial susceptibility profiles, growth curves, biofilm assays, mucoviscosity measurements, and raw survival data from Galleria mellonella and mouse infection experiments for three blaNDM-21-positive ST35 Klebsiella pneumoniae strains (HS898, HS910, HS915).Contents and MethodsGenomic dataIllumina NovaSeq 6000 350-bp paired-end + Nanopore PromethION 10-kb long reads; hybrid assembly with Unicycler.Deposited in NCBI under BioProject PRJNA1265502; accession numbers CP192299–CP192302.Phenotypic experimentsVitek-2 Compact GN334 MIC values.24-h growth curves (96-well, OD600 every 0.5 h, triplicate).Sedimentation ratio (SR) for mucoviscosity.Crystal-violet biofilm quantification (OD595).G. mellonella killing assay: 20 larvae/group, survival recorded every 6 h for 72 h; raw death times (h).Mouse survival: 5 mice/group, challenge 10^6 CFU, survival every 6 h for 72 h; raw death times (h).Organ bacterial loads: spleen, kidney, liver, lung at 24 h post-infection (CFU/organ, n=3).Original H&E histology whole-slide images.Bioinformatics filesAmino-acid sequences of NDM-1 to NDM-21.Neighbour-joining tree file (.meg).cgSNP and cgMLST distance matrices.Linear plasmid comparison SVG (pHS898-3 vs IncX3 reference).