Effect of isoginkgetin on targeting MTHFD2 to induce ROS-mediated apoptosis in non-small cell lung cancer

Purpose: This study investigated the antitumor activity of isoginkgetin in NSCLC and explored its molecular targets and underlying mechanisms.Methods: Cell-based assays were performed to evaluate the effects of isoginkgetin on NSCLC cell growth, migration, apoptosis, and epithelial-mesenchymal transition (EMT). Transcriptome profiling and redox analyses were conducted to assess mechanistic pathways. Target identification involved in silico screening, cellular thermal shift assay, with functional validation through Methylenetetrahydrofolate dehydrogenase 2 (MTHFD2) overexpression. Antitumor efficacy was further examined in NSCLC xenograft models.