Single embryo RNA-seq analysis in RIF1 depleted preimplantation mouse embryos

RNAseq datasets from control and RIF1-depleted mouse embryos to address whether RIF1 depletion results in changes in gene expression. siRNA was microinjected at the zygote stage and controls (embryos injected with siRNA control) and RIF1-depleted embryos (embryos injected with siRNA against Rif1) were collected either at the 4-cell or at the 8-cell stage. RNAseq was performed using SMART-Seq2 in single embryos. Overall design: siRNA for Rif1 or control siRNA in 4-cell and 8-cell stage embryo.