Influence of the Digestion Technique, Protease, and Missed Cleavage Peptides in Protein Quantitation.

Data from ProteomeXchange, PXD ID: PXD001187. Enzyme: LysC_Trypsin, file: 121219_S_CCES_01_03_LysC_Try_1to10_Mixt_1_3.mzXML. Published as part of J Proteome Res. 2014 Jul 28 . From the Abstract: {{i}} Here we evaluated both in-solution and filter-aided digestion protocols and assessed their influence in the estimation of protein abundances using five E. coli mixtures with known amounts of spiked proteins. We observed that replicates of trypsin specificity digestion protocols are highly reproducible in terms of peptide quantitation, with digestion technique and the chosen proteolytic enzyme being the major sources of variability in peptide quantitation. {{/i}}