CIL:39732, Chlorocebus sabaeus, kidney cell. In Cell Image Library
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Isolated nuclei were prepared from COS-7 African Green Monkey and polynucleosomes released after treatment with micrococcal nuclease. Samples were fixed with 0.1% glutaraldehyde for 4 hr at 4C, placed on holey carbon films, vitrified by plunge freezing, and observed in the frozen hydrated state using a Philips CM10 TEM operated at 60KV. Low dose images were recorded on Kodak SO163 film. See also: J Bednar et al. 1998 Nucleosomes, linker DNA and linker histone form a unique structural motif that directs the higher-order folding and compaction of chromatin. Proc Natl Acad Sci USA 95:14173-14178.
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2023-06-28



