Effect of platelet-derived microparticles on the colorectal cancer-vascular endothelium crosstalk - data from in vitro and in vivo study.

The aim of the research was to verify the effect on platelet-derived microparticles (PMP) on the interaction between colorectal cancer cells (CRC) and vascular endothelium.Raw data have been deposited including: (i) Effect of PMP on the adhesion of fluorescently labelled CRC cells to monolayers of HMEC-1 cells in the presence of incorporation inhibitor Dynasore. CRC cells alone, HMEC-1 cells alone or both CRC cells and HMEC-1 cells were preincubated with PMP and with or without the addition of the dynamine inhibitor, Dynasore. The data are presented as the number of adhered, fluorescently labeled CRC cells; (ii) The effect of PMP and conditioned medium of PMP-stimulated CRC cells on the integrity of the HMEC-1 cell monolayer. The data are presented as the area covered with fluorescently labelled HMEC-1 cells; (iii) Permeability of the PMP-stimulated HMEC-1 monolayer for the passage of Dextran-FITC. The data are presented as fluorescence values measured at 488 nm; (iv) The mRNA expression of intercellular junction proteins (PECAM-1, ZO-1 and VE-cadherin) in HMEC-1 cells treated with PMP or conditioned medium of PMP-stimulated CRC cells. The data are presented as the fold change in relative to that of unstimulated cells; (v) Expression of PECAM-1 and ZO-1 on the surface of CRC cells incubated with PMP or with conditioned medium of PMP-stimulated CRC cells. The data are presented as the MFI values; (vi) Normalised densitometric values of PECAM-1 and ZO-1 expression in PMP-stimulated HMEC-1 cells, in relative to those of unstimulated cells; (vii) Migration of fluorescently labelled CRC cells through monolayer of PMP-stimulated HMEC-1 cells; (viii) The data are presented as number of migrated fluorescently labeled CRC cells; (ix) Concentrations of total MMP-2 and human MMP-9 in the plasma obtained from mice treated with selected lines of CRC cells and intravenously injected with PMP. The data are presented as ng/ml of MMP-2/MMP-9 in plasma; (x) Expression of CD62P and αIIbβ3 on resting and thrombin-stimulated platelets obtained from a CRC mouse model intravenously injected with PMP. The data are presented as a percentage of CD62P- or αIIbβ3-positive platelets; (xi) Quantified data of the adhesion of fluorescently labelled HT29, SW480 and SW620 cells to monolayers of HMEC-1 cells. CRC cells alone, HMEC-1 cells alone or both CRC cells and HMEC-1 cells were preincubated with PMP. The data are presented as the number of adhered, fluorescently labeled CRC cells; (xii) The effect of Dynasore on the incorporation of PMP into CRC cells; (xiii) The effect of PMPs on the level of plasma inflammatory markers in CRC in vivo model. The data are presented as pg/ml of MCP-1, TNF-α, IFN-γ, IL-10 and IL-6 in plasma from CRC mouse model intravenously injected with PMPs; (xiv) Size distribution of PMPs isolated from thrombin-stimulated platelets, measured by using NTA (Nanoparticle Tracking Analysis).Data are presented in ODS files. Each ODS file refers to separate figure presented in the publication linked to these data.