Single-cell sequencing of 12 PBMC samples to study the different immune response after SARS-CoV-2 vaccines injection. Single-cell sequencing of 12 PBMC samples to study the different immune response after SARS-CoV-2 vaccines injection

The effectiveness of SARS-CoV-2 vaccines varies among individuals. In the COVID-19 global pandemic, it showed a significant Th1 characteristics, suggesting that the immune disorder and production of SARS-CoV-2 antibodies may be related to Th1/Th2 bias. However, the molecular mechanisms of how Th1/Th2 bias affects the differences in host immune responses to viruses remain unclear. A total of 3 subjects with elevated antibody (E) and 3 without changes (C) were recruited. We collected peripheral blood and performed single-cell sequencing before and after the third dose of SARS-CoV-2 vaccine. The changes of T cell clusters were analyzed and differential analysis of T cells between the two groups was performed. Overall design: Six subjects with different antibody levels after vaccination (three with markedly elevated vs. three without) were enrolled in this study. All subjects were vaccinated with inactivated SARS-CoV-2 vaccine. Peripheral blood samples were collected before and after the third dose of the vaccine. In total, 12 peripheral blood samples (3 pre-injection samples in the elevated group (E), 3 post-injection samples in E group, 3 pre-injection samples in the control group (C), 3 post-injection samples in C group) were sequenced. *************************************************************** Submitter states that missing raw data are being made available for controlled access in the Genome Sequence Archive (https://ngdc.cncb.ac.cn/gsa/). ***************************************************************