Dionaea muscipula DYSCALCULIA cultivar vs WT RNAseq after mechanostimulation

The Venus Flytrap 'DYSCALCULIA' (DYSC) naturally occurring mutant / cultivar refers to the originally called 'ERROR' cultivar discovered by Mathias Maier in 2011 (Bailey & McPherson, Dionaea, pg. 214, 2013). This cultivar is not able to shut its traps after touching the trigger hairs two times, as a normal wild type (WT) does. To understand the underlying molecular basis of the mutant's disorder we sequenced trap RNA one hour after the application of mechanostimulation. The mechanostimulation treatment was done by touching the trigger hairs ten times, every time inducing one action potential (AP). In total, ten APs were elicited (1 AP every minute). Same procedure was done in parallel to the functional WT - which was used as a reference when comparing the two phenotypes. As we know, the stress hormone, jasmonic acid (JA), is synthesized in touched traps. The levels of JA was measured in both phenotypes after touch stimulation and wounding. Additionally, the molecular mimic of JA-ile, in the form of 0.1 mM coronatine (COR), was sprayed on the open traps of both phenotypes and harvested after one hour, followed by RNA-seq. For all RNA-seq experiments (paired-end, with a sequencing length of 150 bp and a sequencing depth of more than 20 mil. sequences) three replicates were used for each group.