DeepBacs – Bacillus subtilis denoising dataset

Live-cell time series of vertically aligned <em>B. subtilis</em> cells expressing FtsZ-GFP protein fusion. Additional information can be found in this github wiki. The example shows raw and denoised images (Noise2Void 2D) of vertically aligned <em>B. subtilis</em> cells (VerCINI) <strong>Data type</strong>: Fluorescence images of vertically oriented <em>B. subtilis </em>cells <strong>Microscopy data type</strong>: 2D widefield images (fluorescence) <strong>Microscope</strong>: Custom-built 100x inverted microscope bearing a 100x TIRF objective (Nikon CFI Apochromat TIRF 100XC Oil); images were captured on a Prime BSI sCMOS camera (Teledyne Photometrics) <strong>Cell type</strong>: <em>B. subtilis</em> strain SH130 grown under agarose pads, Cells were imaged at 1 frame/second with continuous exposure for 2 minutes at 1-8 W/cm2 <strong>File format</strong>: .tiff (16-bit) One frame was selected from each time series <strong>Image size</strong>: 1024x1024 px² (Pixel size: 65 nm) <strong>Author(s)</strong>: Mia Conduit¹, Séamus Holden^1,2 <strong>Contact email</strong>: [e-mail] <strong>Affiliation</strong>: 1) Centre for Bacterial Cell Biology, Biosciences Institute, Newcastle University, NE2 4AX UK 2) ORCID: 0000-0002-7169-907X <strong>Associated publications</strong>: Whitley <em>et al</em>., 2021, Nature Communications, https://doi.org/10.15252/embj.201696235