Inhibition of PARP activity does not affect transcription of Dnmt1, but influences its promoter acetylation level.

(A) Real time qRT-PCR analysis of mRNA accumulation for Dnmt1. Histogram indicates mRNA level of cells treated with PJ34 for 1 h (black), relative to untreated cells (grey, value ~1). Values were normalized with Actβ. (B) Real time qRT-PCR analysis of histone H3 and H4 acetylation of two Dnmt1 promoter regions. Histograms indicate acetylation level ofcells treated with PJ34 for 1 h (black), relative to untreated cells (grey, value ~1). Values were normalized with an internal region of Actβ. Primers A1 and A2 were described in Materials and Methods. Error bars indicate the standard deviation of data obtained from three independent experiments. NT: control cells, no treatment. *p ≤ 0.05; **p ≤ 0.01.