Figure_3.jpg

A) Cell viability after seven days (% of control FBS-free medium) for both experimental conditions: #1) LPS concentrations diluted in FBS-free α-MEM (basal medium); or #2) LPS concentrations diluted in α-MEM + 10% FBS (growth medium). Columns are means and error bars are standard deviations (n=8). Uppercase letters compare the different conditions and lowercase letters compare the LPS concentrations within the same experimental condition. Distinct letters are statistically different (two-way ANOVA/Sidak, α=5%). B) Cell viability after 21 days (% of control FBS-free medium) for both experimental conditions. Columns are means and error bars are standard deviations (n=8). Distinct letters compare each column, showing statistical differences (two-way ANOVA/Sidak, α=5%). C) Mineralized matrix formation (% of control FBS-free medium) for both experimental conditions. Columns are means and error bars are standard deviations (n=8). Distinct letters compare each column, showing statistical differences (two-way ANOVA/Sidak, α=5%).