Atlas of Gene Expression at Microanatomical Resolution of the Submandibular Salivary Gland

The goal of this study was to generate a spatio-temporal atlas of gene expression in the developing salivary gland. This will allow us to identify changes in gene expression that may result in the formation of the different domains of the salivary gland. At least 4 microarrays per location per time point were collected. The originating samples are rather small, so many clefts, ducts and buds had to be pooled to generate enough RNA for hybridization.本研究旨在构建发育中唾液腺的基因表达时空图谱（spatio-temporal atlas）。该图谱将助力我们识别可能介导唾液腺不同结构域形成的基因表达变化。每个时间点的每个采样位点至少采集4份基因芯片样本。由于起始样本体量极小，需混合大量腺裂隙、导管及腺芽组织，才能获取足够的核糖核酸（RNA）用于芯片杂交实验。