Gene expression profiling in lung and breast cancer cells treated by Bloom-specific siRNAs

A549 and MDA-MB-231 cells were transfected with the Bloom-specific siRNA. Total RNA was extracted using Trizol at 48 hours after transfection. RNA-SEQ was carried out to profile the gene expression in both culture conditions. Overall design: The goal of this experiment was to profie the genes that are regulated by Bloom (BLM RecQ like helicase) in cancer cells. Gene expression was probed globally using RNA-SEQ.