Genome-wide chromatin accessibility profiling in adult human heart using DNase-seq

We performed DNase-seq to identify open chromatin regions in adult human hearts as potential cis-regulatory elements We collected two adult female human heart left ventricle samples, one from an autopsy accessed 5 hours postmortem and one from an explant accessed 12 hours post-surgery, respectively. To evaluate the effect of postmortem or post-surgery time interval on DNaseI hypersensitivity, two pieces were dissected from each sample, both snap frozen but either immediately after dissection or after being left at room temperature for 19 hours and 12 hours, respectively