16S sequencing data of Asian female population with BMD reduction

Background: Osteopenia and osteoporosis are both common bone diseases in postmenopausal women, but their clinical manifestations tend to be unnoticeable until bone loss-induced fractures occur which could endanger personal safety and lead to other injuries. This phenomenon makes it difficult to identify the population with osteoporosis, leading to failure to receive treatment timely. As a significant public health concern, the early diagnosis of osteopenia and osteoporosis has become an urgent clinical topic.
Methods: We conducted metabolomic analysis on plasma samples and 16S sequencing on fecal samples from humans. While 142 women participated in metabolomic analysis, only 118 of them joined the transcriptomics cohort. By statistical methods, we managed to identify relevant biomarkers of osteopenia and osteoporosis consisting of metabolites and genera and establish early diagnosis models, including clinical features, classical indexes and selected biomarkers. The efficiency of these m..., The fecal samples were collected in sterile plastic cups from participants and stored at -80 °C within 1 hour. The microbial DNA was extracted by using TIANamp Stool DNA Kit (TIANGEN, Beijing, China) and the DNA solution was stored at -20°C. The concentration of the extracted DNA was determined by Nanodrop (Spectrophotometer ND1000) for further amplification. The forward Illumine adapter sequence was CCTACGGGNGGCWGCAG and the reverse was GACTACHVGGGTATCTAATCC.
The sequencing results were analyzed according to Qiime2 procedure. Illumina NovaSeq 6000 was used for acquiring the raw sequencing data and DADA2 was applied for filtration and denoising of the raw data which is equivalent to clustering DNA sequences with 10% similarity[26].  Only low-quality sequences were removed and corrected, and algorithm recognition was used to remove chimeras. The redundancy of the denoised sequences was directly removed redundancy to obtain features, including OTUs, ASVs, and other information.
The clear ..., , # 16S sequencing data of Asian female population with BMD reduction

These data files are original data of 16S rRNA sequencing among Asian female of normal group, osteopenia group and osteoporosis group. These data files are original data of 16S rRNA sequencing among Asian females of normal group, osteopenia group and osteoporosis group. The files from A1 to A53 belong to the osteopenia group, the files from B1 to B36 belong to the osteoporosis group and the files from Con1 to Con37 belong to the normal group.