Spatial reconstruction of the early hepatic transcriptomic landscape after an acetaminophen overdose using single-cell RNA sequencing

We leveraged single-cell RNA sequencing to understand the early molecular
events that define the hepatocyte response to acetaminophen exposure at a
subpopulation level. We spatially assigned hepatocytes along the
portol-central vein axis by using established landmark genes. By spatially
assigning the hepatocytes were were able to account for innate differences
in gene expression that existed along this gradient. The excel files
herein provide the full list of differentially expressed genes between key
subpopulations of interest. Additionally, we classified genes as either
pericentral zonated, periportal zonated, or non-zonated, the full list of
genes and their spatial assignments are included in the appropriate excel
file.