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Primary structures of HBD4 and the analogs with net charge at neutral pH.

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a The HBD4 sequence corresponds to the commercially obtained synthetic peptide. The additional EF sequence in analogs corresponds to the start of the mature region of preproHBD4 protein sequence [17]. b Disulfide connectivity in H4-1d, H4-1dΔEF, H4-1d[S] and H4-1d[S]ΔEF is C3-C6. Disulfide connectivities in H4-2d are C2-C4 and C3-C6. Disulfide connectivities in HBD4 and H4-3d are C1-C5, C2-C4, and C3-C6. Side chain protecting groups acetamidomethyl (Acm) and tertiary-butyl (tBu) are shown by subscripts adjacent to cysteines respectively. Cationic residues are underlined. Primary structures of HBD4 and the analogs with net charge at neutral pH.
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