Efect of Arpp21 deficiency on TCR repertoire diversity
收藏NIAID Data Ecosystem2026-05-01 收录
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https://www.ncbi.nlm.nih.gov/sra/SRP425235
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To assess the impact of Arpp21 deficiency on the TCR repertoire, we generated Arpp21-deficient mice from which we isolated CD4 T cells and subjected them to NGS sequencing. Overall design: From the spleens of age-matched mice, 7 of which were WT and 7 of which were Arpp21 KO, 6.8 million CD4 T cells were purified by FACS. Technical details: - Library preparation kit: Takara SMARTer Mouse TCR alpha-beta Profiling Kit - Sample QC method: Qubit and Tapestation - Library QC method: Qubit and Tapestation - Instrument: Illumina MiSeq - Read length: PE300
创建时间:
2024-03-21



