Gene expression analysis of murine splenic IL-10+ and IL-10- B cells

B cells belong to the adaptive immune system and have multiple functions. Besides their well characterized role in the promotion of the immune response, specific B cell subsets were shown to have an anti-inflammatory function. These cells are termed regulatory B cells and they exert their function mainly through the production of interleukin 10 (IL-10). This study focuses on B cells capable of producing IL-10 after 5 hours of stimulation with LPS, PMA and ionomycin, without any distinction based on surface markers. total B cells were stimulated for 5h with LPS, PMA and ionomycin and separated through an IL-10 secretion assay coupled to FACS-sorting.