Genome-wide analysis of transcription start sites and polysome association at each 5''UTR variant in Oryza sativa suspension cells

To determine the 5''UTR sequence and to evaluate the polysome association at each 5''UTR variant, we performed polysome fractionation analysis and cap analysis of gene expression (CAGE). Using sucrose density ultra centrifugation, polysomal fraction RNA and total fraction RNA were collected from control suspension cells and heat stressed suspension cells in Oryza sativa. Afterwards, nAnT-iCAGE libraries were generated from purified RNAs.