Inducible promoters are one of cellular and molecular biology’s most important technical tools. The ability to deplete, replete, and overexpress genes on demand is the foundation of most functional st
BACKGROUND: The DNA-dependent RNA polymerase from T7 bacteriophage (T7 RNAP) has been extensively characterized, and like other phage RNA polymerases it is highly specific for its promoter. A combined
A fusion of the HNH domain of CasDinG from Sulfitobacter sp. JL08 (SsCasDinG) to the characterized type IV-A1 CasDinG of Pseudomonas oleovorans (PoCasDinG) was created. An engineered catalytic active
