Microarray data for microRNA-196a KD in immortalised abdominal and gluteal pre-adipocytes.

Microarray data for pre-adipocyte cell lines imAPAD-mir-196aKD, imAPAD-Con, imGPAD-mir196aKD and imGPAD-Con. Six replicates of passage 10 imAPAD mir-196aKD, imAPAD-Con, imGPAD mir-196aKD and imGPAD-Con pre-adipocytes were harvested in Tri-reagent. Cells were harvested on day 3 after plating and were sub-confluent. RNA was isolated using the mirVana miRNA Isolation Kit (Life Technologies). RNA concentration was assessed using the Nanodrop ND-1000 spectrophotometer and by RiboGreen® assay and samples were normalised to give 100ng RNA in a total volume of 11 μl. RNA quality was verified using the Agilent TapeStation (Agilent, Santa Clara, US). RNA samples were reverse transcribed using the Illumina TotalPrep-96 RNA Amplification Kit and then converted into labelled cDNA using the Illumina Whole-Genome Gene Expression Direct Hybridisation Assay. The labelled ss-cDNA was then hybridised to two HumanGene2.1 ST-16 Array Plates. The array was washed, stained and scanned using the Affymetrix GeneTitan platform. Raw data were Robust multi-array (RMA) normalised and checked for quality. Differential expression analysis was performed in R.