Identification of phosphorylation sites in human IGF2BP1 by immunoprecipitation coupled to mass spectrometry

Many RNA-binding proteins are regulated by posttranslational modifications, most commonly phosphorylation. IGF2BP1 is an RNA-binding protein, which regulates protein stability, translation and localization. Moreover, it was proposed to regulate mRNA stability and translation during proteotoxic stress. Here, we used immunoprecipitation coupled to mass spectrometry to identify phosphorylation sites in IGF2BP1 under control conditions and under conditions where mammalian cells were subjected to proteotoxic stress.