Peanut callus RNA sequencing data

Prolonged expression of gene-editing components in CRISPR-modified plants can compromise trait stability and increase the risk of off-target mutations. To mitigate these issues in peanut (Arachis hypogaea), we explored the use of callus-specific promoters to restrict Cas9 expression to the callus stage, minimizing its activity in regenerated plants. To identify callus-specific promoters, we performed RNA sequencing (RNA-seq) on T5 and T11 generation callus derived from the peanut cultivar YH9326.