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Kinetic constants for wt Mtb Pck in the presence of different divalent cations.

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https://figshare.com/articles/dataset/_Kinetic_constants_for_wt_Mtb_Pck_in_the_presence_of_different_divalent_cations_/1350588
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aFor gluconeogenic reaction 100 mM HEPES-NaOH, pH 7.2, 0.3 mM OAA, 0.2 mM GTP, 2 mM MgCl2, 0.2 mM MnCl2, 10 mM DTT, 10 U/ml LDH, 3 U/ml PK and 0.2 mM NADH was used. bFor anaplerotic reaction 100 mM HEPES-NaOH, pH 7.2, 100 mM KHCO3, 37 mM DTT, 2 mM PEP, 1 mM GDP, 2 mM MgCl2, 0.1 mM MnCl2, 2 U/ml MDH and 0.25 mM NADH was used. n.d.: Kinetic constants cannot be calculated. Kinetic constants for wt Mtb Pck in the presence of different divalent cations.
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2015-03-23
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