AP-1 inhibition in DRG neurons by A-Fos dominant negative construct

Prior analysis suggsted that that AP-1 TFs play a role in expression of specific B2-SINE following in-vivo nerve injury. We thus sought to experimnetally test the role of AP-1 transcription factors in upregulation of B2-SINE RNA expression in DRG neurons. Neurons were transduced with PHP.S-AAV vectors expressing either A-Fos (dominant negative construct that targets Fos-interacting transcription factors), GFP control or were left untransduced. RNA was extracted 7 days later and analyzed to identify effects on B2-SINE expression and known AP-1 targetted mRNAs. Overall design: Primary cultures from neuron-enriched DRG tissues were transduced using sensory-neuron specific AAV vector (PHP.S). Experimental conditions were DN (A-Fos AP-1 dominant negative), GFP control and untransduced control. Experiment replicates are cultures from separate mice and were divided between the 3 experimental conditions.