Effects of AR-42 Treatment on Gene Expression in the Zebrafish Tail Fin Amputation (TFA) Model

We investigated the inflammatory environmental effects of AR-42 on neutrophil recruitment by performing an RNA-seq analysis with tail tissue in the zebrafish TFA model. The RNA-seq results showed that AR-42 modulates cytokine/chemokine signaling in the local inflammatory environment. For RNA sequencing (RNA-seq) analysis, the 3-dpf WT zebrafish larvae receiving DMSO or 10 μM AR-42 treatments were categorized into 4 groups: ① DMSO treated larvae without TFA modeling (referred to as DMSO), ② AR-42 treated larvae without TFA modeling (AR-42), ③ DMSO treated larvae with TFA modeling (DMSO_TFA) and ④ AR-42 treated larvae with TFA modeling (AR-42_TFA). And there are four biological replicates in each group. After tail fin amputation, larvae were immediately treated with DMSO or 10 μM AR-42 for 1 hr before sampling. Tail tissues posterior to the cloaca in each larva were collected (n = 150) and immediately froze in liquid nitrogen before RNA isolation.