Single cell RNA Sequencing analysis on CD45 positive cells isolated form Lewis Lung Carcinoma (LLC) tumors.

The goal of this study is to compare transcriptome profiling of different populations of CD45 immune cells of isolated from LLC tumors from miR-21 conditionally deleted on LysM expressing cells (mir-21 flox/flox; lysmCre) mice vs. control (mir-21flox/flox) mice. Overall design: LLC cells were subqutaneously implated in 8 weeks old control (mir-21flox/flox) or miR-21 conditionally deleted on LysM expressing cells (mir-21 flox/flox; lysmCre) mice. After 14 days tumors were isolated and collagenase diggested then Cd45 positive cells were FACS sorted and RNA was straced for subsequent RNA-seq analysis.