Exploration of genes involved in mature B cells-directed inflammation caused by PTEN mutation

To expose additional genes and signaling pathways involved in the B cell-mediated inflammation in CD23-cKO mice, we performed single-cell RNA-sequencing (scRNA-seq) transcriptomic analysis. These data suggest that the induction of TLR9-mediated IL-6 production triggered by PTEN deficiency relies on BCR-directed endocytosis. Splenic B cells from CD23-cre-control, CD23-cKO, mb-1-cre control and mb-1-cKO mice were left unstimulated (resting), stimulated with TLR9 ligand CpG-ODN1668, or with CpG-ODN1668 plus SB203580 for 2h. The cells were labeled with BD™ Abseq antibody-oligo-conjugates B220, CD19, IgD, CD21, CD23, CD5, CD40, CD80, CD86, CD95, CD138, MHCII. The RNA libraries were prepared by using BD Rhapsody™ WTA Reagent Kit 4 pack (Cat#633802).