High temporal resolution transcriptomic profiling from fertilization through ZGA

Single murine embryo RNA-seq covering fertilization through ZGA sampled every 2 hours after fertilization Female C57BL/6J mice were superovulated with PMSG and HCG 46hrs apart. Females were then paired with C57BL/6J stud mice. Sample collection began 12hrs post HCG from females that had been plugged at least 2hrs prior and was done every 2hrs spanning the 1C - 2C transition. Embryos were lysed and stored separately according to SMART-SEQ v4 manufacturer instructions. Once all samples were collected, cDNA synthesis and amplification and cleanup were done on the whole cohort according to manufacturer's instructions. Embryos from 0hrs post fertilization to late 2C were processed as a cohort. Oocyte and Morula samples were processed in separate batches.