CBTi-seq validates specialized alternative splicing patterns in gene-edited cells

we compared the ability to reveal cell-specific splicing patterns between knockdown and control groups using CBTi-seq and 3' targeting methods. The numbers of detected genes showed no significant difference between the two methods. However, gene body coverage distribution indicated differential genomic coverage patterns which CBTi-seq shows a uniform full-length coverage. CBTi-seq robustly identified pronounced exon skipping events spanning exon 4 to exon 6 in the knockdown group, contrasting with the constitutive splicing pattern observed in controls. Overall design: establish Rrm2-knockdown and control cell groups, and then sequenced by CBTi-seq and 3' targeting methods, respectively