Genome-wide analysis of mechano-responsive gene expression of mesenchymal stem cells after laminar shear stress. Genome-wide analysis of mechano-responsive gene expression of mesenchymal stem cells after laminar shear stress

Analysis of mechano-regulation of mesenchymal stem cell gene expression level. The hypothesis tested in the present study was that laminar shear stress influences the cytoskeleton arrangement and amount and then triggers seriers of cell metabolism and cellular functions. Results provide important information of the response of mesenchymal stem cell to laminar shear stress, such as specific mechano-responsive genes, up- or down-regulated specific anabolic/catabolic cellular functions. Overall design: Total RNA obtained from mesenchymal stem cells subjected to 15 min, 30 min, 45 min, 60 min, 4 hours in vitro larminar shear stress compared to control mesenchymal stem cells.