Genome-wide analysis of gene expression by LSD1 overexpression or inhibiting by ORY1001 with or without irradiation in MDA-MB-231 breast cancer cells

Analysis of LSD1 regulation after DNA damage at gene expression level.We supposed that LSD1 is involved in Double Strand DNA repair, alterated LSD1 expression with transfection vector or LSD1 inhibtor in MDA-MB-231, and finally found LSD1 promted DNA damage reponse after IR treatment. Total RNA obtained from MDA-MB-231 cells with six groups:WTIR-,WTIR+,OE+IR-,OE+IR+,ORY1001+IR-,ORY1001+IR+,IR represents exposed with ionization radiation (IR) treatment, WT represents control without any treatment, OE represents LSD1 overexpression (OE) with transfected LSD1 vector, ORY1001 represents treatment with LSD1 inhibitor ORY1001.