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Gene expression data of OsHMGB707 overexpression and RNAi transgenic rice plants under normal conditions

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NIAID Data Ecosystem2026-03-12 收录
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE130821
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资源简介:
High mobility group (HMG) proteins play an important role in regulation of gene transcription through modulate the structure of DNA. In this study, OsHMGB707, a HMG gene localized in rice drought resistance QTL interval, was isolated and the function on rice stress resistance was identified. Overexpression of OsHMGB707 significantly enhanced the drought resistance of the transgenic rice plants, whereas the OsHMGB707-RNAi transgenic rice plants exhibited slightly decrease in drought stress tolerance. To search the downstream genes regulated by OsHMGB707, we performed microarray analysis of the OsHMGB707-overexpressing, OsHMGB707-RNAi and wild-type plants under both normal conditions using Affymetrix Rice Genome Genechip. 21-day-old plants of the OsHMGB707-overexpressing line OE1, OsHMGB707-RNAi line RNAi1 as well as the wild-type plants were used in the normal condition. The OsHMGB707-overexpression plants,RNAi plants and the wild-type plants were cultured in the culture solution for 21 days.The leaves of the plants were sampled for RNA isolation, three biological replicate samples each from the OsHMGB707 overexpression, OsHMGB707 RNAi and wild type lines were profiled using rice whole-genome microarrays.
创建时间:
2021-08-24
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