RNA-seq analysis for wild-type fibroblasts and patient fibroblasts bearing the m.3243A>G mutatioin

The goal of this study is to compare RNA-seq of wild-type fibroblasts and patient fibroblasts bearing the m.3243A>G mutatioin. When comparing patient fibroblasts to wild-type ones and using a significance level of false discovery rate (FDR) < 0.05, we identified 3394 transcripts of which 1849 were upregulated and 1545 were downregulated. Overall design: Three replicates for each fibroblast line (passage 4). L1084, Control 1; L1028,Control 2; L1007, Patient 1; L1008, Patinet 2. Control fiborblasts sometimes refer to as wild-type or WT. Similarly Patient fiborblasts sometimes refer to as mutant or MT.