Differential expression of cell cycle proteins on treatment with hUCBSC.

Human cell cycle cDNA arrays (SA Biosciences, Cat. No. PAHS-020) were
run using cDNA from single and co-cultures of glioma cells with
hUCBSC, as per manufacturer’s instructions as explained in
Materials and Methods. Real
time PCR was carried out and changes in gene expression were
illustrated as fold increase/decrease according to
manufacturer’s instructions. The cut-off induction determining
expression was 2.0 or –2.0 fold changes. Genes that met these
criteria were considered to be upregulated or downregulated.