Differential expression of cell cycle proteins on treatment with hUCBSC.

Human cell cycle cDNA arrays (SA Biosciences, Cat. No. PAHS-020) were run using cDNA from single and co-cultures of glioma cells with hUCBSC, as per manufacturer’s instructions as explained in Materials and Methods. Real time PCR was carried out and changes in gene expression were illustrated as fold increase/decrease according to manufacturer’s instructions. The cut-off induction determining expression was 2.0 or –2.0 fold changes. Genes that met these criteria were considered to be upregulated or downregulated.