Additional file 1: of Biofilm production by Haemophilus influenzae and Streptococcus pneumoniae isolated from the nasopharynx of children with acute otitis media

Supplementary Data S1: Different cut-offs used to determine the biofilm production by S. pneumoniae: the different cut-offs used were as followed where AB represented the stained wells containing attached bacteria, CW the stained control wells containing bacteria-free medium only and G the bacterial growth control i) AB – CW: strong production > 0.30, moderate production 0.10–0.30, negative 6, moderate production 2–6, negative 1.10, moderate production 0.35–1.10, negative