Gene expression profile at single cell levels of the cells from the controls and tau P251L Drosophila brain.

Tau protein has been implicated in the pathology of Alzheimer's disease and related disorders. We CRISPR engineered an endogenous model of tauopathy by knocking in a pathogenic human tau mutation, tau P301L, in Drosophila, tau P251L KI. We used single cell RNA sequencing to identify the differentially expressed genes or pathways. Control and tau P251L KI brains were dissociated, cells were isolated and analyzed using scRNAseq.