Analysis of local glucocorticoid receptor occupancy at glucocorticoid-repressed inflammatory enhancers in Beas-2B airway epithelial cells

This study combined ChIP-seq using two different GR antibodies and stable GR knockdown in Beas-2B airway epithelial cells to determine whether glucocorticoid-mediated repression of inflammatory enhancers requires local GR occupancy Duplicate samples of Beas-2B cells transduced with lentiviral-shRNA against GR (shGR) or a control construct (shCtrl) were treated with vehicle (ethanol), TNF (20 ng/ml), dexamethasone (100 nM) or TNF+dex for one hour prior to chromatin immunoprecipitation with one of two GR antibodies and sequencing. Duplicate Input DNA samples, representing both shCtrl and shGR groups, were included as controls.