Effect of Top2a depletion on the transcripional profile of HPV31-positive HaCaT (HaCaT31)cells

To explore the downstream targets of Top2a and the potential mechanisms of how TOP2A regulates HPV replication, RNA-seq was applied to HPV-positive cells with Top2a knockdown. Overall design: HaCaT31 cells were transduced with viruses expressing Top2a shRNA and subsequently selected for stable expression of the shRNA using puromycin. The knockdown cells and the scramble cells were then compared to identify potential downstream targets of Top2a.