Mean percent mortality and larval weight (± SE) of the D. virgifera virgifera when exposed to native and iodinated Cry34Ab1/35Ab1, Cry6Aa and Cry3Aa for 5 days on overlay diet bioassay.

aCry protein followed by the letter (i) denotes that the protein was iodinated. Unlabelled Cry proteins were in native form. Cry34/35Ab1 was comprised of full length Cry34Ab1 and Cry35Ab1 chymotrypsin-resistant core, whereas full length Cry6Aa and Cry3Aa1 trypsin-resistant core were used.bFor each Cry protein, means followed by the same letter within the column are not significantly different at Pr = 0.05. For statistical analysis data were grouped by Cry protein and corresponding buffer. Statistical analysis on the mortality count data was performed using Chi-square Likelihood Ratio test. Analysis of variance (ANOVA) with means comparison was conducted using Tukey-Kramer HSD test on larval weight data (4 replications, n = 20). For Cry3A, similar statistical analyses were used except there were 3 replications, n = 16.